The Saints have beaten Chichester City and last Sunday they were narrowly beaten by second-placed Forest Green Rovers.
Manager Steve Robertson said: "After a run of away games, the team are determined to maintain their top position but they were not expecting an easy game at Southampton where, in the past, we have had difficulty collecting points."
The Town have included Chenise Austin in Sunday’s squad having recently signed her from Cheltenham Town
SQUAD: Robyn Levett, Laura Morgan, Beth Thompson, Lisa Coe, Sam Quinn, Michelle Rich, Chyna Collings, Alice Smith, Sarah Uzzell, Megan Attenborough, Ellie Maynard, Kelly Nightingale Chenise Austin & Sally Butterfield.